Stem Cell Therapy
What is a stem cell?
Stem cells are the source of every cell, tissue and organ in our bodies. Stem cells that are the source of the cells consisting our body; they renew the tissues that are deformed, tired, damaged or sickened by aging. Stem cells have the ability to regenerate themselves and the ability to turn into many different cells of the organism.
Where do stem cells come from?
The most widely used source for them has been bone marrow until now. Bone marrow stem cells are the ancestors of the cells in the blood system and they differentiate, proliferate and grow in order to become circulating cells in the blood.
To classify, the sources of stem cells can be listed as follows:
Embryonic stem cells
Fetal stem cells
Adult stem cells
What are the potential uses of stem cells?
Differentiated cells in our body can not be renewed by natural means if they are seriously damaged or become ill. Stem cells are used instead of these diseased or damaged cells to produce healthy and functional cells. The process of replacing a diseased cell with a healthy cell called “cell therapy”, which is similar to organ transplantation, the only difference is the transferring the cells instead of organs. In general, researchers are exploring the potential of grown, fetal, and embryonic stem cells to be a source of specific cells such as nerve cells, muscle cells, blood cells, and skin cells that are used to treat various diseases.
The object in regenerative medicine:
Repair or improve the functioning of damaged tissues and organs due to aging, disease or trauma. Today, the definition of regenerative medicine is used to describe stem cell research and therapies used to restore function of tissues or organs. This treatment can be done in two ways: specialized cells from the stem cells can be obtained in the labs or drugs can be given to activate the stem cells in the tissues and a faster, more efficient repair can be achieved.
Use of fibroblasts as specialized cells from stem cells in laboratories
What is Fibroblast:
Fibroblasts are cells predominant in connective tissue structure and having many roles in the skin. It is responsible for the synthesis of intercellular matrix proteins. In addition, they play a role in many physiological processes in the stroma of tissues or organs through cytokines and growth factors which they secrete. Fibroblasts, which play an important role in deep structure and integrity of the skin, have tissue regenerating and rejuvenating effects. Collagen, the most abundant protein in the skin, plays an important role in ensuring skin integrity because of its unique physical structure. It is known that when the collagen-degrading enzymes synthesized by fibroblasts are increased, structural abnormalities develop and the signs of aging appear.
Fibroblasts, which have an effective role in wound healing, in promoting and supporting the deep cellular structure, are now frequently used for skin rejuvenation and treatment of skin damages. It can be used to treat chronic wounds, scars in the form of hollows that can occur after acne, or chicken pox, burns, trauma, post-surgery cavities and scarring, lip augmentation, removal of wrinkles in the face.
Autologous (obtained from the person) fibroblast injections are used to treat problems such as wrinkles, stains on the skin, and maturation in the skin color that occur with age. The fibroblast cells injected into the skin begin to produce collagen and elastin by passing through the area where they are transplanted, thus regulate the protein structure in the skin structure. Thus, it shows a strong anti-aging feature on the skin by removing the signs of aging from the skin.
Especially acne scars that occur on the face are an important cosmetic problem. In dermato-cosmetology, fillers are used for a long time for acne scars. However, in these treatments, it is necessary to repeat the applications due to product destruction after a while. Because of side effects that may occur, such products are rarely used in acne scars. In the treatment of acne scars, autologous fibroblast injections can be performed alone or in combination with applications such as lasers and dermarols. Skin biopsy specimens taken from the treated patients showed no increase in the thickness of the dermis and fibroblast volume as well as no inflammation.
Patien- specific fibroblast production:
3 mm punch biopsy sample is taken from an area of the patient that cannot be exposed to sunlight such as back of the ear etc. Biopsy material as well as blood must be taken from the patient in order to make the product specific to the individual. The biopsy material taken in a sterile solution and the blood taken from the patient are delivered to the laboratory. Fibroblast cells are first isolated from biopsy material in our laboratory, which is under international standards (cGMP conditions) and have production permit by the Ministry of Health. Subsequently, these fibroblast cells are proliferated/produced under sterile conditions. The production process takes about 4-6 weeks. At the end of the process, the number of fibroblast cells that can be transferred to the individual is reached. After the quality control and characterization stages, the product is presented in vials to be transferred to the patient under special conditions.
Injections can be made in 3-4 sessions with 15-30 days intervals through classical mesotherapy technique. No anesthesia is used for administration. During application, cells can also be combined with different fillers.